Genes expressed in Brugia malayi infective third stage larvae.
Identifieur interne : 00BD24 ( Main/Exploration ); précédent : 00BD23; suivant : 00BD25Genes expressed in Brugia malayi infective third stage larvae.
Auteurs : M L Blaxter [Royaume-Uni] ; N. Raghavan ; I. Ghosh ; D. Guiliano ; W. Lu ; S A Williams ; B. Slatko ; A L ScottSource :
- Molecular and biochemical parasitology [ 0166-6851 ] ; 1996.
Descripteurs français
- KwdFr :
- ADN complémentaire, Amorces ADN, Animaux, Banque de gènes, Brugia malayi (génétique), Brugia malayi (physiologie), Caenorhabditis elegans (génétique), Données de séquences moléculaires, Filariose lymphatique, Gènes d'helminthe, Humains, Introns, Larve, Macaca, Rats, Réaction de polymérisation en chaîne, Régulation de l'expression des gènes au cours du développement, Signaux de triage des protéines (biosynthèse), Similitude de séquences d'acides aminés, Similitude de séquences d'acides nucléiques, Séquence d'acides aminés, Séquence nucléotidique.
- MESH :
- biosynthèse : Signaux de triage des protéines.
- génétique : Brugia malayi, Caenorhabditis elegans.
- physiologie : Brugia malayi.
- ADN complémentaire, Amorces ADN, Animaux, Banque de gènes, Données de séquences moléculaires, Filariose lymphatique, Gènes d'helminthe, Humains, Introns, Larve, Macaca, Rats, Réaction de polymérisation en chaîne, Régulation de l'expression des gènes au cours du développement, Similitude de séquences d'acides aminés, Similitude de séquences d'acides nucléiques, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Brugia malayi (genetics), Brugia malayi (physiology), Caenorhabditis elegans (genetics), DNA Primers, DNA, Complementary, Elephantiasis, Filarial, Gene Expression Regulation, Developmental, Gene Library, Genes, Helminth, Humans, Introns, Larva, Macaca, Molecular Sequence Data, Polymerase Chain Reaction, Protein Sorting Signals (biosynthesis), Rats, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid.
- MESH :
- chemical , biosynthesis : Protein Sorting Signals.
- chemical : DNA Primers, DNA, Complementary.
- genetics : Brugia malayi, Caenorhabditis elegans.
- physiology : Brugia malayi.
- Amino Acid Sequence, Animals, Base Sequence, Elephantiasis, Filarial, Gene Expression Regulation, Developmental, Gene Library, Genes, Helminth, Humans, Introns, Larva, Macaca, Molecular Sequence Data, Polymerase Chain Reaction, Rats, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid.
Abstract
We have used a tag sequencing approach to survey genes expressed in the third stage infective larvae of the human filarial nematode parasite Brugia malayi. RNA was isolated from late vector-stage L3 larvae after days 9 or 10 of infection in mosquitos, and converted to cDNA by reverse transcriptase. Double-stranded cDNA was produced by either conventional methods (non-SL cDNA library) or by PCR using the nematode spliced leader (SLI) and oligo(dT) primers (SL cDNA library). Two clone libraries (one from SL and one from non-SL cDNAs) were constructed in lambda ZapII. A set of these full-length clones was selected and 596 inserts were sequenced from the 5' end. We have identified 364 B. malayi genes (the majority of which are new) that encode housekeeping proteins, structural proteins, proteins of immediate immunological or drug-discovery interest as well as a large class of novel sequences which may prove to have significant involvement in host invasion. Extensive, genome-wide approaches to the analysis of larval gene expression are now possible for B. malayi. We present several examples of this approach.
PubMed: 8784774
Affiliations:
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Raghavan</name></author><author><name sortKey="Ghosh, I" sort="Ghosh, I" uniqKey="Ghosh I" first="I" last="Ghosh">I. Ghosh</name></author><author><name sortKey="Guiliano, D" sort="Guiliano, D" uniqKey="Guiliano D" first="D" last="Guiliano">D. Guiliano</name></author><author><name sortKey="Lu, W" sort="Lu, W" uniqKey="Lu W" first="W" last="Lu">W. Lu</name></author><author><name sortKey="Williams, S A" sort="Williams, S A" uniqKey="Williams S" first="S A" last="Williams">S A Williams</name></author><author><name sortKey="Slatko, B" sort="Slatko, B" uniqKey="Slatko B" first="B" last="Slatko">B. Slatko</name></author><author><name sortKey="Scott, A L" sort="Scott, A L" uniqKey="Scott A" first="A L" last="Scott">A L Scott</name></author></analytic><series><title level="j">Molecular and biochemical parasitology</title><idno type="ISSN">0166-6851</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Brugia malayi (genetics)</term><term>Brugia malayi (physiology)</term><term>Caenorhabditis elegans (genetics)</term><term>DNA Primers</term><term>DNA, Complementary</term><term>Elephantiasis, Filarial</term><term>Gene Expression Regulation, Developmental</term><term>Gene Library</term><term>Genes, Helminth</term><term>Humans</term><term>Introns</term><term>Larva</term><term>Macaca</term><term>Molecular Sequence Data</term><term>Polymerase Chain Reaction</term><term>Protein Sorting Signals (biosynthesis)</term><term>Rats</term><term>Sequence Homology, Amino Acid</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire</term><term>Amorces ADN</term><term>Animaux</term><term>Banque de gènes</term><term>Brugia malayi (génétique)</term><term>Brugia malayi (physiologie)</term><term>Caenorhabditis elegans (génétique)</term><term>Données de séquences moléculaires</term><term>Filariose lymphatique</term><term>Gènes d'helminthe</term><term>Humains</term><term>Introns</term><term>Larve</term><term>Macaca</term><term>Rats</term><term>Réaction de polymérisation en chaîne</term><term>Régulation de l'expression des gènes au cours du développement</term><term>Signaux de triage des protéines (biosynthèse)</term><term>Similitude de séquences d'acides aminés</term><term>Similitude de séquences d'acides nucléiques</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Protein Sorting Signals</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term><term>DNA, Complementary</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Signaux de triage des protéines</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term><term>Caenorhabditis elegans</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Brugia malayi</term><term>Caenorhabditis elegans</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Elephantiasis, Filarial</term><term>Gene Expression Regulation, Developmental</term><term>Gene Library</term><term>Genes, Helminth</term><term>Humans</term><term>Introns</term><term>Larva</term><term>Macaca</term><term>Molecular Sequence Data</term><term>Polymerase Chain Reaction</term><term>Rats</term><term>Sequence Homology, Amino Acid</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term><term>Amorces ADN</term><term>Animaux</term><term>Banque de gènes</term><term>Données de séquences moléculaires</term><term>Filariose lymphatique</term><term>Gènes d'helminthe</term><term>Humains</term><term>Introns</term><term>Larve</term><term>Macaca</term><term>Rats</term><term>Réaction de polymérisation en chaîne</term><term>Régulation de l'expression des gènes au cours du développement</term><term>Similitude de séquences d'acides aminés</term><term>Similitude de séquences d'acides nucléiques</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have used a tag sequencing approach to survey genes expressed in the third stage infective larvae of the human filarial nematode parasite Brugia malayi. RNA was isolated from late vector-stage L3 larvae after days 9 or 10 of infection in mosquitos, and converted to cDNA by reverse transcriptase. Double-stranded cDNA was produced by either conventional methods (non-SL cDNA library) or by PCR using the nematode spliced leader (SLI) and oligo(dT) primers (SL cDNA library). Two clone libraries (one from SL and one from non-SL cDNAs) were constructed in lambda ZapII. A set of these full-length clones was selected and 596 inserts were sequenced from the 5' end. We have identified 364 B. malayi genes (the majority of which are new) that encode housekeeping proteins, structural proteins, proteins of immediate immunological or drug-discovery interest as well as a large class of novel sequences which may prove to have significant involvement in host invasion. Extensive, genome-wide approaches to the analysis of larval gene expression are now possible for B. malayi. We present several examples of this approach.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country><region><li>Écosse</li></region><settlement><li>Édimbourg</li></settlement><orgName><li>Université d'Édimbourg</li></orgName></list><tree><noCountry><name sortKey="Ghosh, I" sort="Ghosh, I" uniqKey="Ghosh I" first="I" last="Ghosh">I. Ghosh</name><name sortKey="Guiliano, D" sort="Guiliano, D" uniqKey="Guiliano D" first="D" last="Guiliano">D. Guiliano</name><name sortKey="Lu, W" sort="Lu, W" uniqKey="Lu W" first="W" last="Lu">W. Lu</name><name sortKey="Raghavan, N" sort="Raghavan, N" uniqKey="Raghavan N" first="N" last="Raghavan">N. Raghavan</name><name sortKey="Scott, A L" sort="Scott, A L" uniqKey="Scott A" first="A L" last="Scott">A L Scott</name><name sortKey="Slatko, B" sort="Slatko, B" uniqKey="Slatko B" first="B" last="Slatko">B. Slatko</name><name sortKey="Williams, S A" sort="Williams, S A" uniqKey="Williams S" first="S A" last="Williams">S A Williams</name></noCountry><country name="Royaume-Uni"><region name="Écosse"><name sortKey="Blaxter, M L" sort="Blaxter, M L" uniqKey="Blaxter M" first="M L" last="Blaxter">M L Blaxter</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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